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Genotyping by ligation assays.

Current protocols in human genetics - 1 May 2001

Nickerson D A, Ankener W, Delahunty C, Kwok P Y

Abstract excerpt

This unit describes two methods of genotyping DNA sequences containing known nucleotide variations. The first protocol describes a colorimetric method for genotyping DNA samples amplified by the polymerase chain reaction (PCR) using an oligonucleotide ligation assay (OLA). The second protocol describes the ligase chain reaction (LCR), a method for simultaneously amplifying and genotyping genomic DNA samples. A...

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PMID: 18428270
DOI: 10.1002/0471142905.hg0206s10

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