Article
A mass spectrometry-based multiplex SNP genotyping by utilizing allele-specific ligation and strand displacement amplification.
Biosensors & bioelectronics - 15 May 2017
Park Jung Hun, Jang Hyowon, Jung Yun Kyung, Jung Ye Lim, Shin Inkyung, Cho Dae-Yeon, Park Hyun Gyu
Abstract excerpt
We herein describe a new mass spectrometry-based method for multiplex SNP genotyping by utilizing allele-specific ligation and strand displacement amplification (SDA) reaction. In this method, allele-specific ligation is first performed to discriminate base sequence variations at the SNP site within the PCR-amplified target DNA. The primary ligation probe is extended by a universal primer annealing site while the...
Topics
- Alleles
- Biosensing Techniques
- Breast Neoplasms
- Female
- Genes, BRCA1
- Genotype
- Genotyping Techniques
- Humans
- Nucleic Acid Amplification Techniques
- Polymorphism, Single Nucleotide
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization