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Application of a double-reporter-guided mutant selection method to improve clavulanic acid production in Streptomyces clavuligerus.

Metabolic engineering - 1 Jan 2000

Xiang Si-Hai, Li Jia, Yin Hua, Zheng Jian-Ting, Yang Xu, Wang Hai-Bin, Luo Jia-Li, Bai Hua, Yang Ke-Qian

Abstract excerpt

A reporter-guided mutant selection (RGMS) method has been developed wherein reporters are used to facilitate selection of target over-expressing mutants. It was applied to improve clavulanic acid (CA) production in Streptomyces clavuligerus. In a single-reporter design, the transcriptional activator ccaR of CA biosynthesis was chosen as the over-expressing target, and neo (resistance to kanamycin) as the...

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PMID: 19584003
DOI: 10.1016/j.ymben.2009.06.003

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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