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Article

Development of a colorimetric assay for rapid quantitative measurement of clavulanic acid in microbial samples.

Science China. Life sciences - 1 Feb 2012

Dai Xida, Xiang Sihai, Li Jia, Gao Qiang, Yang Keqian

Abstract excerpt

We developed a colorimetric assay to quantify clavulanic acid (CA) in culture broth of Streptomyces clavuligerus, to facilitate screening of a large number of S. clavuligerus mutants. The assay is based on a β-lactamase-catalyzed reaction, in which the yellow substrate nitrocefin (λ (max)=390 nm) is converted to a red product (λ (max)=486 nm). Since CA can irreversibly inhibit β-lactamase activity, the level of...

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PMID: 22415687
DOI: 10.1007/s11427-012-4287-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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