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Targeted activation of silent natural product biosynthesis pathways by reporter-guided mutant selection.

Metabolic engineering - 1 Mar 2015

Guo Fang, Xiang Sihai, Li Liyuan, Wang Bin, Rajasärkkä Johanna, Gröndahl-Yli-Hannuksela Kirsi, Ai Guomin, Metsä-Ketelä Mikko, Yang Keqian

Abstract excerpt

The continuously increasing genome sequencing data has revealed numerous cryptic pathways, which might encode novel secondary metabolites with interesting biological activities. However, utilization of this hidden potential has been hindered by the observation that many of these gene clusters remain silent (or poorly expressed) under laboratory conditions. Here we present reporter-guided mutant selection (RGMS)...

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PMID: 25554073
DOI: 10.1016/j.ymben.2014.12.006

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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