Article
A mitotic recombination map proximal to the APC locus on chromosome 5q and assessment of influences on colorectal cancer risk.
BMC medical genetics - 10 Jun 2009
Howarth Kimberley, Ranta Susanna, Winter Eitan, Teixeira Ana, Schaschl Helmut, Harvey John J, Rowan Andrew, Jones Angela, Spain Sarah, Clark Susan, Guenther Thomas, Stewart Aengus, Silver Andrew, Tomlinson Ian
Abstract excerpt
BACKGROUND: Mitotic recombination is important for inactivating tumour suppressor genes by copy-neutral loss of heterozygosity (LOH). Although meiotic recombination maps are plentiful, little is known about mitotic recombination. The APC gene (chr5q21) is mutated in most colorectal tumours and its usual mode of LOH is mitotic recombination. METHODS: We mapped mitotic recombination boundaries ("breakpoints")...
Topics
- Cell Line, Tumor
- Chromosome Mapping
- Chromosomes, Human, Pair 5
- Colorectal Neoplasms
- Genes, APC
- Genetic Predisposition to Disease
- Humans
- Loss of Heterozygosity
- Microsatellite Repeats