Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Evaluation of transcriptional activity of p53 in individual living mammalian cells.

Analytical biochemistry - 15 Apr 2009

Imagawa Toshiaki, Terai Tomoko, Yamada Yoshifumi, Kamada Rui, Sakaguchi Kazuyasu

Abstract excerpt

To estimate the transcriptional activity of p53 in individual living mammalian cells, we constructed the enhanced green fluorescent protein-red fluorescent protein (EGFP-DsRed) reporter system with the EGFP-p53 expression vector and the reporter plasmid, which carried a p53-dependent promoter. The expression level and transcriptional activity of EGFP-p53 were determined simultaneously by green and red...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Green Fluorescent Proteins
Share to Luminescent Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutation

Share to Protein Structure, Tertiary

Share to Red Fluorescent Protein

Share to Transcription, Genetic

Share to Tumor Suppressor Protein p53

Source

Identifiers

PMID: 19454241
DOI: 10.1016/j.ab.2009.01.030

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evaluation of transcriptional activity of p53 in individual living mammalian cells.PMID 19454241Read ↗

Improving Reporter Gene Assay Methodology for Evaluating the Ability of Compounds to Restore P53 Activity.Semantic proximity Read ↗

Imaging transcriptional regulation of p53-dependent genes with positron emission tomography in vivoSemantic proximity Read ↗

Easy detection of GFP-positive mutants following forward mutations at specific gene locus in cultured human cells.Semantic proximity Read ↗

Stable formation of mutated p53 multimers in a Chinese hamster cell line causes defective p53 nuclear localization and abrogates its residual function.Semantic proximity Read ↗

Transcriptional properties of feline p53 and its tumour-associated mutants: a yeast-based approach.Semantic proximity Read ↗

How many mutant p53 molecules are needed to inactivate a tetramer?Semantic proximity Read ↗

Single cell monitoring of growth arrest and morphological changes induced by transfer of wild-type p53 alleles to glioblastoma cells.Semantic proximity Read ↗

A functional screen for germ line p53 mutations based on transcriptional activation.Semantic proximity Read ↗

Is green fluorescent protein toxic to the living cells?Semantic proximity Read ↗

The p53 codon 249 mutant--derived from human functional adrenal tumors--can modify the cell shape of normal adrenocortical transfected cells.Semantic proximity Read ↗

Disruption of functions of wild-type p53 by hetero-oligomerization.Semantic proximity Read ↗

Human p53 binds DNA as a protein homodimer but monomeric variants retain full transcription transactivation activity.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.