Article
Functional mapping of anthrax toxin lethal factor by in-frame insertion mutagenesis.
The Journal of biological chemistry - 25 Oct 1991
Quinn C P, Singh Y, Klimpel K R, Leppla S H
Abstract excerpt
Linker insertion mutagenesis was employed to create structural disruptions of the lethal factor (LF) protein of anthrax toxin to map functional domains. A dodecameric linker was inserted at 17 blunt end restriction enzyme sites throughout the gene. Paired MluI restriction sites within the linker allowed the inserts to be reduced from four to two amino acids. Shuttle vectors containing the mutated genes were...
Topics
- Antigens, Bacterial
- Bacterial Toxins
- Base Sequence
- Binding, Competitive
- Genes, Bacterial
- Molecular Sequence Data
- Mutagenesis, Insertional
- Phenotype
- Plasmids
- Restriction Mapping