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Article

Residue histidine 669 is essential for the catalytic activity of Bacillus anthracis lethal factor.

Journal of bacteriology - 1 Nov 2010

Cao Sha, Guo Aizhen, Wu Gaobing, Liu Ziduo, Chen Wei, Feng Chunfang, Zhang Cheng-Cai, Chen Huanchun

Abstract excerpt

The lethal factor (LF) of Bacillus anthracis is a Zn(2+)-dependent metalloprotease which plays an important role in anthrax virulence. This study was aimed at identifying the histidine residues that are essential to the catalytic activities of LF. The site-directed mutagenesis was employed to replace the 10 histidine residues in domains II, III, and IV of LF with alanine residues, respectively. The cytotoxicity...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 20833809
DOI: 10.1128/JB.00485-10
PMCID: PMC2953669

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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