Article
Residue histidine 669 is essential for the catalytic activity of Bacillus anthracis lethal factor.
Journal of bacteriology - 1 Nov 2010
Cao Sha, Guo Aizhen, Wu Gaobing, Liu Ziduo, Chen Wei, Feng Chunfang, Zhang Cheng-Cai, Chen Huanchun
Abstract excerpt
The lethal factor (LF) of Bacillus anthracis is a Zn(2+)-dependent metalloprotease which plays an important role in anthrax virulence. This study was aimed at identifying the histidine residues that are essential to the catalytic activities of LF. The site-directed mutagenesis was employed to replace the 10 histidine residues in domains II, III, and IV of LF with alanine residues, respectively. The cytotoxicity...
Topics
- Animals
- Antigens, Bacterial
- Bacillus anthracis
- Bacterial Toxins
- Gene Expression Regulation, Bacterial
- Histidine
- Mice
- Models, Molecular
- Mutagenesis, Site-Directed
- Mutation
- Protein Binding
- Protein Conformation
- Protein Structure, Tertiary
- Spleen