Article
Secretion of human epidermal growth factor from Saccharomyces cerevisiae using synthetic leader sequences.
Gene - 15 Oct 1991
Clements J M, Catlin G H, Price M J, Edwards R M
Abstract excerpt
We have investigated different leader sequences for their ability to direct the efficient secretion of human epidermal growth factor (hEGF) from Saccharomyces cerevisiae. We designed a consensus signal sequence which directs secretion of hEGF from yeast as efficiently as the yeast invertase signa...
Topics
- Amino Acid Sequence
- Base Sequence
- Consensus Sequence
- DNA Mutational Analysis
- Epidermal Growth Factor
- Genes, Synthetic
- Glycoside Hydrolases
- Glycosylation
- Humans
- Mating Factor
- Molecular Sequence Data
- Mutation
- Peptides
- Plasmids
- Precipitin Tests
- Proprotein Convertases
- Protein Sorting Signals
- Recombinant Fusion Proteins