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Article

In-vitro processing of yeast alpha-factor leader fusion proteins using a soluble yscF (Kex2) variant.

Applied microbiology and biotechnology - 1 Sept 1991

Seeboth P G, Heim J

Abstract excerpt

The Saccharomyces cerevisiae KEX2 gene encodes the membrane-bound endoprotease yscF, which is responsible for the site-specific endoproteolytic cleavages at pairs of basic amino acid residues in the alpha-factor precursor. In order to obtain soluble yscF activity, a mutant KEX2 gene lacking 600 bp coding for the C-terminal 200 amino acids was constructed. Expression of the truncated KEX2 gene in yeast led to the...

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PMID: 1367896
DOI: 10.1007/BF00169893

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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In-vitro processing of yeast alpha-factor leader fusion proteins using a soluble yscF (Kex2) variant.PMID 1367896Read ↗

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