Article
In-vitro processing of yeast alpha-factor leader fusion proteins using a soluble yscF (Kex2) variant.
Applied microbiology and biotechnology - 1 Sept 1991
Seeboth P G, Heim J
Abstract excerpt
The Saccharomyces cerevisiae KEX2 gene encodes the membrane-bound endoprotease yscF, which is responsible for the site-specific endoproteolytic cleavages at pairs of basic amino acid residues in the alpha-factor precursor. In order to obtain soluble yscF activity, a mutant KEX2 gene lacking 600 b...
Topics
- Colorimetry
- Fermentation
- Fungal Proteins
- Hirudins
- Immunoblotting
- Insulin-Like Growth Factor I
- Mating Factor
- Mutation
- Peptides
- Plasmids
- Proprotein Convertases
- Protein Precursors
- Recombinant Fusion Proteins
- Restriction Mapping
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Serine Endopeptidases
- Solubility