Article
A novel Kex2 enzyme can process the proregion of the yeast alpha-factor leader in the endoplasmic reticulum instead of in the Golgi.
Biochemical and biophysical research communications - 28 Feb 1992
Chaudhuri B, Latham S E, Helliwell S B, Seeboth P
Abstract excerpt
The prepro sequence of the yeast prepro-alpha-factor, usually referred to as the alpha-factor leader, has often been used for the efficient secretion of heterologous proteins from the yeast Saccharomyces cerevisiae. The alpha-factor leader consists of a 19-amino acid N-terminal pre or signal sequence followed by a 66-amino acid proregion. After removal of the signal sequence during membrane translocation, the...
Topics
- Base Sequence
- Endoplasmic Reticulum
- Fungal Proteins
- Glycosylation
- Golgi Apparatus
- Molecular Sequence Data
- Mutation
- Proprotein Convertases
- Protein Precursors
- Recombinant Fusion Proteins