Article
Efficient secretion of human parathyroid hormone by Saccharomyces cerevisiae.
Gene - 15 Jun 1990
Gabrielsen O S, Reppe S, Saether O, Blingsmo O R, Sletten K, Gordeladze J O, Høgset A, Gautvik V T, Alestrøm P, Oyen T B
Abstract excerpt
A cDNA encoding mature human parathyroid hormone (hPTH) was expressed in Saccharomyces cerevisiae, after fusion to the prepro region of yeast mating factor alpha (MF alpha). Radioimmunoassay showed high levels of hPTH immunoreactive material in the growth medium (up to 10 micrograms/ml). More than 95% of the immunoreactive material was found extracellularly as multiple forms of hormone peptides. Three internal...
Topics
- Adenylyl Cyclases
- Amino Acid Sequence
- Base Sequence
- Gene Expression
- Humans
- Mating Factor
- Molecular Sequence Data
- Mutation
- Parathyroid Hormone
- Peptides
- Plasmids