A mutant Kex2 enzyme with a C-terminal HDEL sequence releases correctly folded human insulin-like growth factor-1 from a precursor accumulated in the yeast endoplasmic reticulum.

doi:10.1111/j.1432-1033.1992.tb17484.x

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Article

A mutant Kex2 enzyme with a C-terminal HDEL sequence releases correctly folded human insulin-like growth factor-1 from a precursor accumulated in the yeast endoplasmic reticulum.

European journal of biochemistry - 15 Dec 1992

Chaudhuri B, Latham S E, Stephan C

Abstract excerpt

Mutations in the pro region of the yeast DNA hybrid of prepro-alpha-factor and human insulin-like growth factor-1 (IGF-1) cause the accumulation, in the yeast Saccharomyces cerevisiae, of an unglycosylated precursor protein where the pre sequence is missing. The prepro sequence of the prepro-alpha-factor consists of a pre or signal sequence and a proregion which possesses three sites for N-glycosylation....

Topics

Amino Acid Sequence
Base Sequence
Blotting, Western
Endoplasmic Reticulum
Fungal Proteins
Genetic Variation
Humans
Insulin-Like Growth Factor I
Kinetics
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis, Site-Directed

Oligodeoxyribonucleotides

Proprotein Convertases

Protein Precursors

Recombinant Fusion Proteins

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Sequence Deletion

Source

Open in PubMed

Identifiers

PMID: 1483466
DOI: 10.1111/j.1432-1033.1992.tb17484.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1483466-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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A mutant Kex2 enzyme with a C-terminal HDEL sequence releases correctly folded human insulin-like growth factor-1 from a precursor accumulated in the yeast endoplasmic reticulum.PMID 1483466Read ↗

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