Article
Secretion of heterologous proteins by Aspergillus niger: production of active human interleukin-6 in a protease-deficient mutant by KEX2-like processing of a glucoamylase-hIL6 fusion protein.
Journal of biotechnology - 1 Nov 1993
Broekhuijsen M P, Mattern I E, Contreras R, Kinghorn J R, van den Hondel C A
Abstract excerpt
To develop improved methods for heterologous protein production in Aspergillus niger, we studied the secretion of human interleukin-6 (hIL6). Since in vitro experiments with culture medium revealed that hIL6 was rapidly degraded, several protease-deficient strains of A. niger were isolated and te...
Topics
- Amino Acid Sequence
- Aspergillus niger
- Base Sequence
- Blotting, Northern
- Blotting, Western
- Cloning, Molecular
- DNA, Recombinant
- Humans
- Interleukin-6
- Molecular Sequence Data
- Mutation
- Plasmids
- Proprotein Convertases
- Protein Processing, Post-Translational