Article
Mutations of the alpha-galactosidase signal peptide which greatly enhance secretion of heterologous proteins by yeast.
Gene - 15 May 1991
Hofmann K J, Schultz L D
Abstract excerpt
The Saccharomyces carlsbergensis MEL1 gene encodes alpha-galactosidase (melibiase; MEL1) which is readily secreted by yeast cells into the culture medium. To evaluate the utility of the MEL1 signal peptide (sp) for the secretion of heterologous proteins by Saccharomyces cerevisiae, an expression vector was constructed which contains the MEL1 promoter and MEL1 sp coding sequence (MEL1sp). The coding sequences for...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Western
- Genes, Fungal
- Intercellular Signaling Peptides and Proteins
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Peptides
- Plasmids
- Platelet Aggregation Inhibitors