Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.

doi:10.1002/j.1460-2075.1991.tb04966.x

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Article

Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.

The EMBO journal - 1 Dec 1991

Mello C C, Kramer J M, Stinchcomb D, Ambros V

Abstract excerpt

We describe a dominant behavioral marker, rol-6(su-1006), and an efficient microinjection procedure which facilitate the recovery of Caenorhabditis elegans transformants. We use these tools to study the mechanism of C.elegans DNA transformation. By injecting mixtures of genetically marked DNA mol...

Topics

Animals
Base Sequence
Blotting, Southern
Caenorhabditis
Collagen
DNA
Genetic Markers
Molecular Sequence Data
Mutation
Nucleic Acid Hybridization
Plasmids
Recombination, Genetic
Transfection
Transformation, Genetic

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