Article
High efficiency adenovirus-mediated expression of truncated N-terminal huntingtin fragment (htt552) in primary rat astrocytes.
Acta biochimica et biophysica Sinica - 1 Apr 2009
Wang Linhui, Lin Fang, Wu Junchao, Qin Zhenghong
Abstract excerpt
Huntington's disease (HD) is caused by an expansion of polyglutamine tract in N-terminus of huntingtin (htt). The mutation of htt leads to dysfunction and premature death of striatal and cortical neurons. However, the effects of htt mutation on glia remain largely unknown. This study aimed to establish a glia HD model using an adenoviral vector to express wild-type and mutant N-terminal huntingtin fragment 1-552...
Topics
- Adenoviridae
- Animals
- Animals, Newborn
- Astrocytes
- Blotting, Western
- Cell Survival
- Cells, Cultured
- Cerebral Cortex
- Fluorescent Antibody Technique
- Genetic Vectors
- Huntingtin Protein
- Inclusion Bodies
- Mutation
- Nerve Tissue Proteins
- Nuclear Proteins
- Rats
- Rats, Sprague-Dawley
- Time Factors