Article
Folding kinetics of the human prion protein probed by temperature jump.
Proceedings of the National Academy of Sciences of the United States of America - 7 Apr 2009
Hart Tanya, Hosszu Laszlo L P, Trevitt Clare R, Jackson Graham S, Waltho Jonathan P, Collinge John, Clarke Anthony R
Abstract excerpt
Temperature-jump perturbation was used to examine the relaxation kinetics of folding of the human prion protein. Measured rates were very fast (approximately 3,000 s(-1)), with the extrapolated folding rate constant at approximately 20 degrees C in physiological conditions reaching 20,000 s(-1). By a mutational analysis of core residues, we found that only 2, on the interface of helices 2 and 3, have significant...
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