The G226A mutant of Gs alpha highlights the requirement for dissociation of G protein subunits.

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Article

The G226A mutant of Gs alpha highlights the requirement for dissociation of G protein subunits.

The Journal of biological chemistry - 15 Jan 1992

Lee E, Taussig R, Gilman A G

Abstract excerpt

Adenylylcyclase cannot be activated by hormones or guanine nucleotide analogs in membranes from cells that express the G226A mutant form Gs alpha instead of the wild-type protein. The mutant Gs alpha protein appears incapable of undergoing the conformational change necessary for guanine nucleotide-induced dissociation of the G protein alpha subunit from the beta gamma subunit complex (Miller, R.T., Masters, S.B.,...

Topics

Adenylyl Cyclases
Animals
Base Sequence
Brain
Cattle
Chromatography, Gel
DNA
Electrophoresis, Polyacrylamide Gel
Enzyme Activation
Escherichia coli
Fluorescence
GTP-Binding Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Guanosine 5'-O-(3-Thiotriphosphate)

Hydrolysis

Magnesium

Molecular Sequence Data

Mutation

Protein Conformation

Source

Open in PubMed

Identifiers

PMID: 1730644

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1730644-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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