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Article

Characterization and properties of dominant-negative mutants of the ras-specific guanine nucleotide exchange factor CDC25(Mm).

The Journal of biological chemistry - 17 Dec 1999

Vanoni M, Bertini R, Sacco E, Fontanella L, Rieppi M, Colombo S, Martegani E, Carrera V, Moroni A, Bizzarri C, Sabbatini V, Cattozzo M, Colagrande A, Alberghina L

Abstract excerpt

Ras proteins are small GTPases playing a pivotal role in cell proliferation and differentiation. Their activation depends on the competing action of GTPase activating proteins and guanine nucleotide exchange factors (GEF). The properties of two dominant-negative mutants within the catalytic domains of the ras-specific GEF, CDC25(Mm), are described. In vitro, the mutant GEF(W1056E) and GEF(T1184E) proteins are...

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PMID: 10593969
DOI: 10.1074/jbc.274.51.36656

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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