Article
A high-resolution melting approach for analyzing allelic expression dynamics.
Current issues in molecular biology - 1 Jan 2009
Yuan Jiazheng, Haroon Muhammad, Lightfoot David, Pelletier Yvan, Liu Qiang, Li Xiu-Qing
Abstract excerpt
Single nucleotide polymorphisms (SNPs) are single base pair mutations that provide new approaches to studies of allele transcript abundances. High-resolution DNA melting curve (HRM) analysis using a LightScanner (Hi-Res Melting system with Idaho's LC Green) provides post-PCR detection of mutations and SNPs in genomic DNA. This study investigated whether the HRM analysis can distinguish alleles among potato...
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