Relative quantitation of restriction fragment length polymorphic DNAs via DNA melting analysis provides an effective way to determine allele frequencies.

Abstract excerpt

To accurately and precisely estimate the allele frequencies in DNA pools for a cost-effective approach to correlate genetic variations to phenotypic traits, we exploited differential melting kinetics between restriction fragment length polymorphic DNAs. The allele frequencies of two SNPs in a series of DNA mixtures with known allelic compositions of the SNPs were determined by analyzing the meltings of restricted...

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