Article
Genome-wide SNP discovery in tetraploid alfalfa using 454 sequencing and high resolution melting analysis.
BMC genomics - 6 Jul 2011
Han Yuanhong, Kang Yun, Torres-Jerez Ivone, Cheung Foo, Town Christopher D, Zhao Patrick X, Udvardi Michael K, Monteros Maria J
Abstract excerpt
BACKGROUND: Single nucleotide polymorphisms (SNPs) are the most common type of sequence variation among plants and are often functionally important. We describe the use of 454 technology and high resolution melting analysis (HRM) for high throughput SNP discovery in tetraploid alfalfa (Medicago sativa L.), a species with high economic value but limited genomic resources. RESULTS: The alfalfa genotypes selected...
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