Article
Optimized detection of sequence variation in heterozygous genomes using DNA microarrays with isothermal-melting probes.
Proceedings of the National Academy of Sciences of the United States of America - 26 Jan 2010
Gresham David, Curry Bo, Ward Alexandra, Gordon D Benjamin, Brizuela Leonardo, Kruglyak Leonid, Botstein David
Abstract excerpt
The use of DNA microarrays to identify nucleotide variation is almost 20 years old. A variety of improvements in probe design and experimental conditions have brought this technology to the point that single-nucleotide differences can be efficiently detected in unmixed samples, although developing reliable methods for detection of mixed sequences (e.g., heterozygotes) remains challenging. Surprisingly, a...
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