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Modified High-Resolution Melting (HRM) Marker Systems Increasing Discriminability Between Homozygous Alleles.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023

Watanabe Satoshi, Yamagata Yoshiyuki, Kotoda Nobuhiro

Abstract excerpt

Targeted single-nucleotide polymorphism (SNP) genotyping, especially for functional nucleotide polymorphism, is widely used for current breeding programs in crops. One of the cost- and time-effective approaches for genotyping is high-resolution melting (HRM) analysis for polymerase chain reaction (PCR) amplicons, including target SNP. The reliability of a genotype obtained from an HRM marker depends on the...

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PMID: 36781655
DOI: 10.1007/978-1-0716-3024-2_25

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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