Article
High-throughput, quantitative analyses of genetic interactions in E. coli.
Nature methods - 1 Sept 2008
Typas Athanasios, Nichols Robert J, Siegele Deborah A, Shales Michael, Collins Sean R, Lim Bentley, Braberg Hannes, Yamamoto Natsuko, Takeuchi Rikiya, Wanner Barry L, Mori Hirotada, Weissman Jonathan S, Krogan Nevan J, Gross Carol A
Abstract excerpt
Large-scale genetic interaction studies provide the basis for defining gene function and pathway architecture. Recent advances in the ability to generate double mutants en masse in Saccharomyces cerevisiae have dramatically accelerated the acquisition of genetic interaction information and the biological inferences that follow. Here we describe a method based on F factor-driven conjugation, which allows for...
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