Article
DNA methylation alters the pattern of spontaneous mutation in Escherichia coli cells (mutD) defective in DNA polymerase III proofreading.
Mutation research - 1 Sept 1991
Palmer B R, Marinus M G
Abstract excerpt
We have shown previously that dam mutants of Escherichia coli have a weak mutator phenotype which generates mostly transition mutations in the P22 mnt gene. In contrast, in mutD5 cells, which have a strong mutator phenotype, transversion mutations were the most prevalent. A dam-16 mutD5 strain, defective in both DNA polymerase III associated-proofreading and Dam-directed mismatch repair exhibits a strong mutator...
Topics
- Bacteriophages
- Cloning, Molecular
- DNA Polymerase III
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genes, Viral
- Methylation
- Mutation
- Phenotype
- Plasmids