Article
Cloning and characterization of a DNA fragment that complements the nfxB mutation in Pseudomonas aeruginosa PAO.
FEMS microbiology letters - 15 Mar 1991
Okazaki T, Iyobe S, Hashimoto H, Hirai K
Abstract excerpt
The 2.2-kilobase DNA fragment that can complement the nfxB mutation in Pseudomonas aeruginosa PAO was cloned from chromosomal DNA of P. aeruginosa PAO1. The nfxB mutants show an increase in resistance to quinolones and hypersusceptibility to beta-lactam and aminoglycoside antibiotics. In the muta...
Topics
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA, Bacterial
- Drug Resistance, Microbial
- Electrophoresis, Polyacrylamide Gel
- Genetic Complementation Test
- Mutation
- Norfloxacin
- Phenotype
- Pseudomonas aeruginosa
- Restriction Mapping
- Transformation, Bacterial