Article
Imipenem resistance in pseudomonas aeruginosa PAO: mapping of the OprD2 gene.
Antimicrobial agents and chemotherapy - 1 Apr 1991
Quinn J P, Darzins A, Miyashiro D, Ripp S, Miller R V
Abstract excerpt
Carbapenem antibiotics have been shown to penetrate the outer membrane of Pseudomonas aeruginosa through a unique porin protein, OprD2. We mapped the OprD2 gene by conjugation using plasmid FP2 and by transduction using phage F116L. This gene maps between 71 and 75 min on the PAO1 chromosome.
Topics
- Bacterial Outer Membrane Proteins
- Bacteriophages
- Chromosome Mapping
- Culture Media
- Drug Resistance, Microbial
- Electrophoresis, Polyacrylamide Gel
- Imipenem
- Mutation
- Plasmids
- Pseudomonas aeruginosa
- Transduction, Genetic