Article
Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori.
FEMS microbiology letters - 1 Mar 2005
Chieda Yuuka, Iiyama Kazuhiro, Yasunaga-Aoki Chisa, Lee Jae Man, Kusakabe Takahiro, Shimizu Susumu
Abstract excerpt
To investigate the pathogenicity of Pseudomonas aeruginosa in insects, a gacA mutant of P. aeruginosa PA01 was constructed by site-directed mutagenesis. The mutant was designated as C1. C1 was less virulent to Bombyx mori than the parent strain. To complement the gacA gene, P. aeruginosa C1 was transformed with the broad host range plasmid pJB3Km1 carrying a 3.9-kbp gacA fragment. The expression of the gacA mRNA...
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