Cloning of Bacteroides fragilis plasmid genes affecting metronidazole resistance and ultraviolet survival in Escherichia coli.

doi:10.1016/0147-619x(90)90035-b

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Article

Cloning of Bacteroides fragilis plasmid genes affecting metronidazole resistance and ultraviolet survival in Escherichia coli.

Plasmid - 1 Mar 1990

Wehnert G U, Abratt V R, Goodman H J, Woods D R

Abstract excerpt

Since reduced metronidazole causes DNA damage, resistance to metronidazole was used as a selection method for the cloning of Bacteroides fragilis genes affecting DNA repair mechanisms in Escherichia coli. Genes from B. fragilis Bf-2 were cloned on a recombinant plasmid pMT100 which made E. coli A...

Topics

Bacteroides fragilis
Blotting, Southern
Cloning, Molecular
DNA, Bacterial
Drug Resistance, Microbial
Escherichia coli
Genes, Bacterial
Metronidazole
Phenotype
Plasmids
Restriction Mapping
Ultraviolet Rays

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