Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Evolving Lac repressor for enhanced inducibility.

Protein engineering, design & selection : PEDS - 1 Feb 2009

Satya Lakshmi O, Rao N M

Abstract excerpt

Lactose repressor (LacI) is one of the best studied prokaryotic transcriptional regulatory proteins till date. Detailed structural, biochemical and genetic studies are being carried out on LacI since four decades to understand its ligand binding properties and the basis of allosteric response. We applied directed evolution methods on LacI to generate mutants with altered allosteric properties. After testing...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Allosteric Regulation
Share to beta-Galactosidase
Share to Directed Molecular Evolution

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genetic Techniques

Share to Isopropyl Thiogalactoside

Share to Lac Repressors

Share to Protein Interaction Domains and Motifs

Share to Transcriptional Activation

Source

Identifiers

PMID: 19029094
DOI: 10.1093/protein/gzn069

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Evolving Lac repressor for enhanced inducibility.PMID 19029094Read ↗

Coevolutionary methods enable robust design of modular repressors by reestablishing intra-protein interactions.Semantic proximity Read ↗

Engineering an allosteric transcription factor to respond to new ligands.Semantic proximity Read ↗

Engineering allostery.Semantic proximity Read ↗

Engineering and application of LacI mutants with stringent expressions.Semantic proximity Read ↗

The lactose repressor system: paradigms for regulation, allosteric behavior and protein folding.Semantic proximity Read ↗

LacI strikes a balance between stability and inducibility.Semantic proximity Read ↗

The genotype-phenotype landscape of an allosteric protein.Semantic proximity Read ↗

Operator search by mutant Lac repressors.Semantic proximity Read ↗

Design and application of a lactulose biosensor.Semantic proximity Read ↗

Positions 94-98 of the lactose repressor N-subdomain monomer-monomer interface are critical for allosteric communication.Semantic proximity Read ↗

Adaptive evolution of the lactose utilization network in experimentally evolved populations of Escherichia coli.Semantic proximity Read ↗

Genetic studies of the lac repressor. XIII. Extensive amino acid replacements generated by the use of natural and synthetic nonsense suppressors.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.