Genetic studies of the lac repressor. XIII. Extensive amino acid replacements generated by the use of natural and synthetic nonsense suppressors.

doi:10.1016/0022-2836(90)90126-7

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Article

Genetic studies of the lac repressor. XIII. Extensive amino acid replacements generated by the use of natural and synthetic nonsense suppressors.

Journal of molecular biology - 20 Mar 1990

Kleina L G, Miller J H

Abstract excerpt

We have altered the amino acid sequence of the lac repressor one residue at a time by utilizing a collection of nonsense suppressors that permit the insertion of 13 different amino acids in response to the amber (UAG) codon, as well as an additional amino acid in response to the UGA codon. We use...

Topics

Amino Acid Sequence
Base Sequence
DNA Transposable Elements
Molecular Sequence Data
Mutation
Phenotype
Repressor Proteins
Suppression, Genetic
Transcription Factors

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