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Engineering and application of LacI mutants with stringent expressions.

Microbial biotechnology - 1 Mar 2024

Wu Jieyuan, Liang Chaoning, Li Yufei, Zeng Yueting, Sun Xu, Jiang Peixia, Chen Wei, Xiong Dandan, Jin Jian-Ming, Tang Shuang-Yan

Abstract excerpt

Optimal transcriptional regulatory circuits are expected to exhibit stringent control, maintaining silence in the absence of inducers while exhibiting a broad induction dynamic range upon the addition of effectors. In the Plac /LacI pair, the promoter of the lac operon in Escherichia coli is characterized by its leakiness, attributed to the moderate affinity of LacI for its operator target. In response to this...

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Identifiers

PMID: 38465475
DOI: 10.1111/1751-7915.14427
PMCID: PMC10926051

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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