Article
Engineering an allosteric transcription factor to respond to new ligands.
Nature methods - 1 Feb 2016
Taylor Noah D, Garruss Alexander S, Moretti Rocco, Chan Sum, Arbing Mark A, Cascio Duilio, Rogers Jameson K, Isaacs Farren J, Kosuri Sriram, Baker David, Fields Stanley, Church George M, Raman Srivatsan
Abstract excerpt
Genetic regulatory proteins inducible by small molecules are useful synthetic biology tools as sensors and switches. Bacterial allosteric transcription factors (aTFs) are a major class of regulatory proteins, but few aTFs have been redesigned to respond to new effectors beyond natural aTF-inducer pairs. Altering inducer specificity in these proteins is difficult because substitutions that affect inducer binding...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.