An essential iteron-binding protein required for plasmid R1162 replication induces localized melting within the origin at a specific site in AT-rich DNA.

Abstract excerpt

The R1162-encoded protein RepIB is essential for replication of the plasmid and binds specifically to iterons within the replicative origin. The protein causes the localized melting of DNA (determined by sensitivity to P1 nuclease) at a site within the AT-rich region of the origin, about 60 bp from the iteron binding sites and separated from them by a GC-rich tract. Point mutations have been isolated in the...

Topics

• Adenine
• Base Sequence
• DNA, Bacterial
• DNA-Binding Proteins
• Electrophoresis, Polyacrylamide Gel
• Escherichia coli
• Genes, Bacterial
• Molecular Sequence Data
• Mutation
• Plasmids
• Repetitive Sequences, Nucleic Acid