Article
Multiplex genotyping for thrombophilia-associated SNPs by universal bead arrays.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2009
Bortolin Susan
Abstract excerpt
This chapter describes a method for the multiplex analysis of six biallelic single nucleotide polymorphisms (SNPs) associated with thrombophilia. The method may, however, be adapted for the simultaneous analysis of up to 100 markers (50 biallelic SNPs) in a single reaction. In the method described, the targets of interest are amplified by single-tube multiplex PCR using six primer sets followed by single-tube...
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