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Bi-allelic SNP genotyping using the TaqMan® assay.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Woodward John

Abstract excerpt

With TaqMan(®) technology allele-specific probes are utilized for quick and reliable genotyping of known polymorphic sites. TaqMan assays are robust in genotyping multiple variant types, including single nucleotide polymorphisms, insertions/deletions, and presence/absence variants. To query a single bi-allelic polymorphism, two TaqMan probes labeled with distinct fluorophores are designed such that they hybridize...

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PMID: 24816660
DOI: 10.1007/978-1-4939-0446-4_6

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