Article
Multiplex genotype determination at a large number of gene loci.
Proceedings of the National Academy of Sciences of the United States of America - 19 Mar 1996
Lin Z, Cui X, Li H
Abstract excerpt
To facilitate large-scale genotype analysis, an efficient PCR-based multiplex approach has been developed. For simultaneously amplifying the target sequences at a large number of genetic loci, locus-specific primers containing 5' universal tails are used. Attaching the universal tails to the target sequences in the initial PCR steps allows replacement of all specific primers with a pair of primers identical to...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.