Article
Analytical validation of the tag-it high-throughput microsphere-based universal array genotyping platform: application to the multiplex detection of a panel of thrombophilia-associated single-nucleotide polymorphisms.
Clinical chemistry - 1 Nov 2004
Bortolin Susan, Black Margot, Modi Hemanshu, Boszko Ihor, Kobler Daniel, Fieldhouse Dan, Lopes Eve, Lacroix Jean-Michel, Grimwood Rebecca, Wells Philip, Janeczko Richard, Zastawny Roman
Abstract excerpt
BACKGROUND: We have developed a novel, microsphere-based universal array platform referred to as the Tag-It platform. This platform is suitable for high-throughput clinical genotyping applications and was used for multiplex analysis of a panel of thrombophilia-associated single-nucleotide polymorphisms (SNPs). METHODS: Genomic DNA from 132 patients was amplified by multiplex PCR using 6 primer sets, followed by...
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