Article
Polymorphism analysis and gene detection by minisequencing on an array of gel-immobilized primers.
Nucleic acids research - 15 Sept 1999
Dubiley S, Kirillov E, Mirzabekov A
Abstract excerpt
Two procedures, multibase and multiprimer, have been developed for single nucleotide extension of primers immobilized within polyacrylamide gel pads on a microchip. In the multibase assay, a primer is next to a polymorphic nucleotide; the nucleotide is identified by the specificity with which the primer incorporates fluorescently labeled dideoxyribo-nucleoside triphosphates. In the multiprimer assay, several...
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