Article
A system for specific, high-throughput genotyping by allele-specific primer extension on microarrays.
Genome research - 1 Jul 2000
Pastinen T, Raitio M, Lindroos K, Tainola P, Peltonen L, Syvänen A C
Abstract excerpt
This study describes a practical system that allows high-throughput genotyping of single nucleotide polymorphisms (SNPs) and detection of mutations by allele-specific extension on primer arrays. The method relies on the sequence-specific extension of two immobilized allele-specific primers that differ at their 3'-nucleotide defining the alleles, by a reverse transcriptase (RT) enzyme at optimized reaction...
Read the complete abstract on PubMedTopics
- Alleles
- Bacteriophage T7
- DNA Mutational Analysis
- DNA Primers
- DNA-Directed RNA Polymerases
- Genotype
- Humans