Article
Primer-design for multiplexed genotyping.
Nucleic acids research - 15 Mar 2003
Kaderali Lars, Deshpande Alina, Nolan John P, White P Scott
Abstract excerpt
Single-nucleotide polymorphism (SNP) analysis is a powerful tool for mapping and diagnosing disease-related alleles. Mutation analysis by polymerase-mediated single-base primer extension (minisequencing) can be massively parallelized using DNA microchips or flow cytometry with microspheres as solid support. By adding a unique oligonucleotide tag to the 5' end of the minisequencing primer and attaching the...
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