A study of integrative transformation in Schizosaccharomyces pombe.

doi:10.1007/BF00279526

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

A study of integrative transformation in Schizosaccharomyces pombe.

Molecular & general genetics : MGG - 1 Apr 1993

Grallert B, Nurse P, Patterson T E

Abstract excerpt

Using the one-step gene disruption technique, we studied the effect of various parameters on the disruption frequency (percentage of homologous integrants) and transformation efficiency (number of transformants per microgram of input DNA) of integrative transformation in Schizosaccharomyces pombe...

Topics

Alleles
Blotting, Southern
Chromosomes, Fungal
Cloning, Molecular
DNA, Fungal
Escherichia coli
Gene Deletion
Genes, Fungal
Genetic Techniques
Plasmids
Promoter Regions, Genetic
Recombination, Genetic
Restriction Mapping
Schizosaccharomyces
Transcription, Genetic
Transformation, Genetic

Source

Open in PubMed

Identifiers

PMID: 8479431
DOI: 10.1007/BF00279526

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8479431-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics