Article
A study of integrative transformation in Schizosaccharomyces pombe.
Molecular & general genetics : MGG - 1 Apr 1993
Grallert B, Nurse P, Patterson T E
Abstract excerpt
Using the one-step gene disruption technique, we studied the effect of various parameters on the disruption frequency (percentage of homologous integrants) and transformation efficiency (number of transformants per microgram of input DNA) of integrative transformation in Schizosaccharomyces pombe. We used suc1 as the target gene for disruption and ura4 as the selectable marker. Our results are as follows. 1) Use...
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