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Article

Efficient gene disruption in the high-ploidy yeast Candida utilis using the Cre-loxP system.

Bioscience, biotechnology, and biochemistry - 23 Apr 2009

Ikushima Shigehito, Fujii Toshio, Kobayashi Osamu

Abstract excerpt

In order to take full advantage of the industrially important yeast Candida utilis, we developed a practical recombinant DNA tool for multiple gene disruption in C. utilis based on the Cre-loxP system, which makes possible the reuse of selection markers. For this purpose, two plasmids were constructed: one harbored a heterologous loxP-flanked selection marker cassette carrying the gene responsible for hygromycin...

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Identifiers

PMID: 19352042
DOI: 10.1271/bbb.80799

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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