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Article

Development of an integrative DNA transformation system for the yeast Candida tropicalis.

Journal of bacteriology - 1 Aug 1990

Haas L O, Cregg J M, Gleeson M A

Abstract excerpt

We developed the alkane and fatty-acid utilizing yeast Candida tropicalis as a host for DNA transformations. The system is based on an auxotrophic mutant host of C. tropicalis which is defective in orotidine monophosphate decarboxylase (ura3). The ura3 host was isolated by mutagenesis and a double-selection procedure that combined nystatin enrichment selection and 5-fluoro-orotic acid resistance selection. As a...

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Identifiers

PMID: 2198267
DOI: 10.1128/jb.172.8.4571-4577.1990
PMCID: PMC213290

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Source receipt: pubmed, retrieved 7 Sept 2026.

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