Development of an integrative DNA transformation system for the yeast Candida tropicalis.

doi:10.1128/jb.172.8.4571-4577.1990

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Article

Development of an integrative DNA transformation system for the yeast Candida tropicalis.

Journal of bacteriology - 1 Aug 1990

Haas L O, Cregg J M, Gleeson M A

Abstract excerpt

We developed the alkane and fatty-acid utilizing yeast Candida tropicalis as a host for DNA transformations. The system is based on an auxotrophic mutant host of C. tropicalis which is defective in orotidine monophosphate decarboxylase (ura3). The ura3 host was isolated by mutagenesis and a doubl...

Topics

Candida
Carboxy-Lyases
DNA, Fungal
Genes, Fungal
Genetic Vectors
Mutation
Orotidine-5'-Phosphate Decarboxylase
Plasmids
Restriction Mapping
Saccharomyces cerevisiae
Transformation, Genetic

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