[Isolation of recipient yeast strains for the stable reproduction of 2-micron DNA vectors].

Article

[Isolation of recipient yeast strains for the stable reproduction of 2-micron DNA vectors].

Molekuliarnaia biologiia - 1 Jan 2000

Nevzgliadova O V, Polishchuk A G

Abstract excerpt

Most Leu- clones of yeast transformants (cir0, pJDB219) can stabilize the replication of 2 micron-vectors with REP3, the stability obtained being comparable to the one for the standard cir0 strain. One of the Leu- clones was used to isolate a plasmid with Rep 1.2 functions ("Rep-helper plasmid")....

Topics

DNA Restriction Enzymes
DNA, Fungal
Genetic Vectors
Phenotype
Plasmids
Saccharomyces cerevisiae
Transformation, Genetic

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