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Article

One-step, PCR-mediated, gene disruption in the yeast Hansenula polymorpha.

Yeast (Chichester, England) - 30 Sept 1999

González C, Perdomo G, Tejera P, Brito N, Siverio J M

Abstract excerpt

Previous evidence based on the experience of our laboratory showed that one-step gene disruption in the yeast Hansenula polymorpha is not straightforward. A systematic study of several factors which could affect gene disruption frequency was carried out. We found that the more critical factor affecting one-step gene disruption in H. polymorpha is the length of the target gene region flanking the marker gene....

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Identifiers

PMID: 10509014
DOI: 10.1002/(SICI)1097-0061(19990930)15:13<1323::AID-YEA459>3.0.CO;2-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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