Article
Use of interplasmid recombination to generate stable selectable markers for yeast transformation: application to studies of actin gene control.
Genome - 1 Oct 1990
Hubberstey A V, Wildeman A G
Abstract excerpt
A plasmid recombination system has been developed that relies upon interplasmid exchanges for yeast cell viability. Two types of plasmids, one carrying the LEU2 allele inserted within yeast actin gene sequences and the other carrying 2-microns plasmid DNA and an intact actin gene, were constructe...
Topics
- Actins
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- Escherichia coli
- Gene Expression
- Genetic Markers
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Recombination, Genetic
- Restriction Mapping
- Saccharomyces cerevisiae
- Transformation, Genetic