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Article

Use of interplasmid recombination to generate stable selectable markers for yeast transformation: application to studies of actin gene control.

Genome - 1 Oct 1990

Hubberstey A V, Wildeman A G

Abstract excerpt

A plasmid recombination system has been developed that relies upon interplasmid exchanges for yeast cell viability. Two types of plasmids, one carrying the LEU2 allele inserted within yeast actin gene sequences and the other carrying 2-microns plasmid DNA and an intact actin gene, were constructed. Neither plasmid alone yielded transformants in the haploid Leu- strain AH22, but when cotransformed, a number of...

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PMID: 2262141
DOI: 10.1139/g90-105

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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